• Scientists resurrect ancient enzymes to

    From ScienceDaily@1:317/3 to All on Mon Apr 18 22:30:46 2022
    Scientists resurrect ancient enzymes to improve photosynthesis

    Date:
    April 18, 2022
    Source:
    Cornell University
    Summary:
    A new study describes a breakthrough in the quest to improve
    photosynthesis in certain crops, a step toward adapting plants to
    rapid climate changes and increasing yields to feed a projected
    9 billion people by 2050.



    FULL STORY ==========================================================================
    A Cornell University study describes a breakthrough in the quest to
    improve photosynthesis in certain crops, a step toward adapting plants
    to rapid climate changes and increasing yields to feed a projected 9
    billion people by 2050.


    ==========================================================================
    The study, "Improving the Efficiency of Rubisco by Resurrecting Its
    Ancestors in the Family Solanaceae," published April 15 in Science
    Advances. The senior author is Maureen Hanson, the Liberty Hyde Bailey Professor of Plant Molecular Biology in the College of Agriculture and
    Life Sciences. First author Myat Lin is a postdoctoral research associate
    in Hanson's lab.

    The authors developed a computational technique to predict favorable gene sequences that make Rubisco, a key plant enzyme for photosynthesis. The technique allowed the scientists to identify promising candidate
    enzymes that could be engineered into modern crops and, ultimately,
    make photosynthesis more efficient and increase crop yields.

    Their method relied on evolutionary history, where the researchers
    predicted Rubisco genes from 20-30 million years ago, when Earth's
    carbon dioxide (CO2) levels were higher than they are today and the
    Rubisco enzymes in plants were adapted to those levels.

    By resurrecting ancient Rubisco, early results show promise for developing faster, more efficient Rubisco enzymes to incorporate into crops and
    help them adapt to hot, dry future conditions, as human activities are increasing heat- trapping CO2 gas concentrations in Earth's atmosphere.

    The study describes predictions of 98 Rubisco enzymes at key moments
    in the evolutionary history of plants in the Solanaceae family, which
    include tomato, pepper, potato, eggplant and tobacco. Researchers use
    tobacco as the experimental model for their studies of Rubisco.



    ==========================================================================
    "We were able to identify predicted ancestral enzymes that do have
    superior qualities compared to current-day enzymes," Hanson said. Lin
    developed the new technique for identifying predicted ancient Rubisco
    enzymes.

    Scientists have known that they can increase crop yields by accelerating photosynthesis, where plants convert CO2, water and light into oxygen
    and sugars that plants use for energy and for building new tissues.

    For many years, researchers have focused on Rubisco, a slow enzyme that
    pulls (or fixes) carbon from CO2 to create sugars. Aside from being
    slow, Rubisco also sometimes catalyzes a reaction with oxygen in the
    air; by so doing, it creates a toxic byproduct, wastes energy and makes photosynthesis inefficient.

    Hanson's lab had previously tried to use Rubisco from cyanobacteria
    (blue-green algae), which is faster but also reacts readily with oxygen, forcing the researchers to try to create micro-compartments to protect
    the enzyme from oxygen, with mixed results. Other researchers have
    tried to engineer more optimal Rubisco by making changes in the enzyme's
    amino acids, though little was known about which changes would lead to
    desired results.

    In this study, Lin reconstructed a phylogeny -- a tree-like diagram
    showing evolutionary relatedness among groups of organisms -- of Rubisco,
    using Solanaceae plants.



    ==========================================================================
    "By getting a lot of [genetic] sequences of Rubisco in existing plants,
    a phylogenetic tree could be constructed to figure out which Rubiscos
    likely existed 20 to 30 million years ago," Hanson said.

    The advantage of identifying potential ancient Rubisco sequences is
    that carbon dioxide levels were possibly as high as 500 to 800 parts per million (ppm) in the atmosphere 25 million to 50 million years ago. Today, heat-trapping CO2 levels are rising sharply due to many human activities,
    with current measurements at around 420 ppm, after staying relatively
    constant under 300 ppm for hundreds of millennia until the 1950s.

    Lin, Hanson and colleagues then used an experimental system developed for tobacco in Hanson's lab, and described in a 2020 Nature Plants paper,
    which employs E. coli bacteria to test in a single day the efficacy of different versions of Rubisco. Similar tests done in plants take months
    to verify.

    The team found that ancient Rubisco enzymes predicted from modern-day Solanaceae plants showed real promise for being more efficient.

    "For the next step, we want to replace the genes for the existing
    Rubisco enzyme in tobacco with these ancestral sequences using CRISPR [gene-editing] technology, and then measure how it affects the production
    of biomass," Hanson said. "We certainly hope that our experiments will
    show that by adapting Rubisco to present day conditions, we will have
    plants that will give greater yields." If their method proves successful, these efficient Rubisco sequences could be transferred into crops such
    as tomatoes, as well as those from other plant families, such as soybeans
    and rice.

    The study was funded by the U.S. Department of Energy.


    ========================================================================== Story Source: Materials provided by Cornell_University. Original written
    by Krishna Ramanujan, courtesy of the Cornell Chronicle. Note: Content
    may be edited for style and length.


    ========================================================================== Journal Reference:
    1. Myat T. Lin, Heidi Salihovic, Frances K. Clark, Maureen R. Hanson.

    Improving the efficiency of Rubisco by resurrecting its ancestors
    in the family Solanaceae. Science Advances, 2022; 8 (15) DOI:
    10.1126/ sciadv.abm6871 ==========================================================================

    Link to news story: https://www.sciencedaily.com/releases/2022/04/220418164926.htm

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